Polarity, division and morphogenesis
Keywordscell polarity, division, mitotic spindle orientation, asymmetric cell division, morphogenesis
Questions related to embryo shape or morphogenesis have haunted developmental biologists for decades. We are using advanced methods in live-imaging, genetics and advanced quantitative measurements to understand how the collective movement of thousand of cells can emerge to shape organs and tissues. Our work will foster a comprehensive of the morphogenesis which deregulation is often observed in pathologies.
Questions related to embryo shape or morphogenesis have haunted developmental biologists for decades. Tissue shaping (morphogenesis) emerges from on collective cell dynamics and the interplay between genetics and mechanics of tissues (Heisenberg et Bellaiche, Cell, 2013). We have introduced the Drosophila notum epithelium as a model to study the morphogenesis of proliferative epithelial tissue (Bosvled et al., Science 2012). Using complementary methods (genetics, high resolution live-imaging, opto-genetics, advanced quantitative measurements and modelling), our work aim to:
1. Dissect the molecular mechanisms regulating apical-basal polarity and mitotic spindle orientation during cell division (figure 1)
2. Link the cytoskeleton organization, the cell dynamics and mechanics to the regulation of large-scale tissue shape changes. (figure 2)
3. Determine how gene expression patterns can account for the distinct cell dynamics observed during tissue morphogenesis.
Our work fosters a comprehensive view of morphogenesis by studying this process at different length-scale (cytoskeleton dynamics, cell dynamics, and tissue deformations) and time-scale (tens of seconds to hours) and by focusing on both its genetic and mechanical regulations.